FATTY-ACID-BINDING SITE OF THE MITOCHONDRIAL UNCOUPLING PROTEIN - DEMONSTRATION OF ITS EXISTENCE BY EPR SPECTROSCOPY OF 5-DOXYL-STEARIC ACID

被引：26

作者：

JEZEK, P ^{[1
]}

FREISLEBEN, HJ ^{[1
]}

机构：

[1] UNIV FRANKFURT,GUSTAV EMBDEN ZENTRUM BIOL CHEM,D-60596 FRANKFURT,GERMANY

来源：

FEBS LETTERS | 1994年 / 343卷 / 01期

关键词：

UNCOUPLING PROTEIN; BROWN FAT MITOCHONDRION; H+ TRANSPORT; RECONSTITUTION; FATTY ACID BINDING SITE; 5-DOXYL-STEARIC ACID;

D O I：

10.1016/0014-5793(94)80599-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fatty acid binding site on isolated mitochondrial uncoupling protein (UcP) is demonstrated using EPR spectroscopy of 5-DOXYL-stearic acid (5-SASL), which also activated H+ transport in proteoliposomes containing UcP In the presence of UcP the EPR spectrum showed reproducible broadening of the low field peak as well as an increase in h(+II)/h(+1M) ratio, rotational correlation time and in order parameter. The half-height width of the low field peak was even doubled in the presence of another UcP ligand, GDP. Palmitic acid reversed the effect of 5-SASL and non-ionizable 5-DOXYL-decane did not exhibit it.

引用

页码：22 / 26

页数：5

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