DIAGNOSTICS OF VIRUS PHYTOPATHOGENS FRUIT TREE PLUM POX VIRUS, PRUNUS NECROTIC RINGSPOT VIRUS AND PRUNUS DWARF VIRUS BY BIOLOGICAL AND MOLECULAR DIAGNOSTICS

被引：0
作者：
Rozak, Julius [1 ]
Galova, Zdenka [2 ,3 ]
机构：
[1] Cent Controlling & Testing Inst Agr, Dept Diagnost, Osada 281, Haniska Pri Kosiciach 04457, Slovakia
[2] Slovak Univ Agr, Fac Biotechnol & Food Sci, Dept Biochem & Biotechnol, SK-94976 Nitra, Slovakia
[3] Chem Inst SAV, Ctr Excellence White Green Biotechnol, Bratislava, Slovakia
来源：
JOURNAL OF MICROBIOLOGY BIOTECHNOLOGY AND FOOD SCIENCES | 2013年 / 2卷
关键词：
viruses PPV; PNRSV; PDV; biological diagnostics; molecular diagnostics; RT-PCR;
D O I：
暂无
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The aim of this study was to determine the incidence of viral phytopathogen Plum pox virus, Prunus necrotic ringspot virus and Prunus dwarf virus in selected localities of Slovakia and diagnose them using a molecular and biological methods. Forty samples of fruit trees of the genus Prunus, twenty samples from intensive plantings and twenty samples from wild subject were analysed. Biological diagnostic by using biological indicators Prunus persica cv. GF 305, Prunus serrulata cv. Schirofugen and molecular diagnostic by mRT-PCR were applied. Five samples with Plum pox virus were infected. The two samples positive for Prunus necrotic ringspot virus and one sample for Prunus dwarf virus were confirmed. The two samples were found to be infected with two viruses Prunus necrotic ringspot virus and Prunus dwarf virus. This work focuses on two techniques, their application to the diagnosis of stone fruit viruses and their routinely used for sanitary and certification programmes.
引用
收藏
页码：2108 / 2114
页数：7
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