REAGENTLESS CHEMICALLY-MODIFIED CARBON-PASTE ELECTRODE BASED ON A PHENOTHIAZINE POLYMER DERIVATIVE AND YEAST ALCOHOL-DEHYDROGENASE FOR THE ANALYSIS OF ETHANOL

被引：101
作者：
DOMINGUEZ, E
LAN, HL
OKAMOTO, Y
HALE, PD
SKOTHEIM, TA
GORTON, L
HAHNHAGERDAL, B
机构：
[1] POLYTECH INST NEW YORK, DEPT CHEM, BROOKLYN, NY 11201 USA
[2] MOLTECH CORP, STONY BROOK, NY 11790 USA
[3] LUND UNIV, CTR CHEM, DEPT ANALYT CHEM, S-22100 LUND, SWEDEN
[4] LUND UNIV, CTR CHEM, DEPT APPL MICROBIOL, S-22100 LUND, SWEDEN
来源：
BIOSENSORS & BIOELECTRONICS | 1993年 / 8卷 / 3-4期
关键词：
REAGENTLESS BIOSENSOR; ENZYME ELECTRODE; CHEMICALLY MODIFIED CARBON PASTE ELECTRODE; DEHYDROGENASE; NAD+ NADH; POLYMER BOUND MEDIATOR;
D O I：
10.1016/0956-5663(93)85038-P
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
Amperometric biosensors for ethanol were constructed by immobilizing yeast alcohol dehydrogenase (ADH) on carbon paste (graphite powder: paraffin oil) chemically modified with a polymer to which a necessary mediator for electrocatalytic NADH oxidation had been covalently attached. Three different approaches were tested: adsorption of ADH on the electrode surface and adding NAD+ to the contacting buffer; having both ADH and NAD+ mixed into the paste; and co-immobilizing ADH and NAD+ in the paste by adding polyethylenimine to the reaction mixture. The last approach resulted in the highest and fastest response. When inserted into a flow injection system, the biosensor responded linearly to ethanol between 2 muM and 3 mM. The biosensor operates at +100 mV vs. Ag/AgCl.
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页码：229 / 237
页数：9
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