LIPASE-CATALYZED ACYLATION OF SUGARS SOLUBILIZED IN HYDROPHOBIC SOLVENTS BY COMPLEXATION

被引：96

作者：

IKEDA, I ^{[1
]}

KLIBANOV, AM ^{[1
]}

机构：

[1] MIT,DEPT CHEM,CAMBRIDGE,MA 02139

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1993年 / 42卷 / 06期

关键词：

LIPASE; ORGANIC SOLVENTS; SUGAR ACYLATION; POLYMERIC MATERIALS; SOLUBILIZATION;

D O I：

10.1002/bit.260420616

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

By complexing glucose and other mono- and disaccharides with phenylboronic acid, they were solubilized in many organic solvents. Pseudomonas sp. lipoprotein lipase readily acylated such solubilized sugars in these solvents (no reaction was observed without phenylboronic acid, i.e., when sugars are insoluble in the reaction medium). Solubilized D-glucose was enzymatically acylated with vinyl acrylate on a preparative scale, and the resultant 6-O-acryloylglucose was chemically polymerized to yield a sugar-based polyacrylate with a molecular weight of 14,000 Da. (C) 1993 John Wiley & Sons, Inc.

引用

页码：788 / 791

页数：4

共 22 条

[1] ENZYMATIC-SYNTHESIS OF VARIOUS 1'-O-SUCROSE AND 1-O-FRUCTOSE ESTERS
CARREA, G
RIVA, S
SECUNDO, F
DANIELI, B
[J]. JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1989, (05): : 1057 - 1061
[2] CHOPINEAU J, 1988, BIOTECHNOL BIOENG, V1, P536
[3] ENZYMATIC AND CHEMOENZYMATIC APPROACHES TO POLYMER SYNTHESIS
DORDICK, JS
[J]. TRENDS IN BIOTECHNOLOGY, 1992, 10 (08) : 287 - 293
[4] Ferrier RJ, 1978, ADV CARBOHYD CHEM BI, V35, P31
[5] FORSTNER M, 1989, CARBOHYD RES, V139, P294
[6] Haines A. H., 1976, ADV CARBOHYD CHEM BI, V33, P11, DOI DOI 10.1016/S0065-2318(08)60280-
[7] ENZYMES IN CARBOHYDRATE SYNTHESIS - LIPASE-CATALYZED SELECTIVE ACYLATION AND DEACYLATION OF FURANOSE AND PYRANOSE DERIVATIVES
HENNEN, WJ
SWEERS, HM
WANG, YF
WONG, CH
[J]. JOURNAL OF ORGANIC CHEMISTRY, 1988, 53 (21) : 4939 - 4945
[8] KIMURA S, 1961, MAKROMOLEKUL CHEM, V50, P155
[9] ASYMMETRIC TRANSFORMATIONS CATALYZED BY ENZYMES IN ORGANIC-SOLVENTS
KLIBANOV, AM
[J]. ACCOUNTS OF CHEMICAL RESEARCH, 1990, 23 (04) : 114 - 120
[10] LAITINEN HA, 1975, CHEM ANAL, P361

← 1 2 3 →