INHIBITION OF STIMULATED BONE-RESORPTION INVITRO BY TIMP-1 AND TIMP-2

被引：62

作者：

HILL, PA

REYNOLDS, JJ

MEIKLE, MC

机构：

[1] GUYS HOSP, UNITED MED & DENT SCH, DEPT ORTHODONT & PAEDIAT DENT, LONDON, ENGLAND

[2] UCL EASTMAN DENT HOSP, INST DENT SURG, DEPT ORTHODONT, LONDON WC1X 8LD, ENGLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1177卷 / 01期

基金：

英国医学研究理事会;

关键词：

BONE RESORPTION; METALLOPROTEINASE; ENZYME INHIBITION; TIMP-1; TIMP-2; (OSTEOCLAST);

D O I：

10.1016/0167-4889(93)90159-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant human TIMP-1 and TIMP-2 (tissue inhibitors of metalloproteinases) inhibited bone resorption induced by either parathyroid hormone or 1,25-dihydroxyvitamin D3 in cultured neonatal mouse calvariae. The inhibition was reversible, dose-dependent and complete at 1 mug/ml inhibitor concentration. TIMP-2 was more potent than TIMP-1. TIMP-1 and TIMP-2 also inhibited basal bone resorption. Neither metalloproteinase inhibitor affected protein synthesis, DNA synthesis, the PTH-enhanced secretion of beta-glucuronidase or the spontaneous release of lactate dehydrogenase. These results suggest that endogenous TIMPs play a central role in regulating both physiological and pathological bone resorption.

引用

页码：71 / 74

页数：4

共 22 条

[1] HYPERTROPHIC CHONDROCYTES PRODUCE IMMUNOREACTIVE COLLAGENASE INVIVO [J].