MARKEDLY INCREASED EXPRESSION OF CYTOCHROME-P-450 17-ALPHA-HYDROXYLASE (P-450C17) MESSENGER-RNA IN ADRENOCORTICAL ADENOMAS FROM PATIENTS WITH CUSHINGS-SYNDROME

被引：23
作者：
OGO, A
HAJI, M
OHASHI, M
NAWATA, H
机构：
[1] Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka
来源：
MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1991年 / 80卷 / 1-3期
关键词：
CUSHINGS SYNDROME; STEROIDOGENESIS; P-450SCC MESSENGER RNA; P-450C17 MESSENGER RNA; P-450C21 MESSENGER RNA; P-450C11 MESSENGER RNA;
D O I：
10.1016/0303-7207(91)90145-I
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
We studied the contents of cortisol (F) and dehydroepiandrosterone (DHEA) and the expression of mRNA of cytochrome P-450 for side-chain cleavage (P-450scc), 17-alpha-hydroxylase (P-450c17), 21-alpha-hydroxylase (P-450c21) and 11-beta-hydroxylase (P-450c11) in adrenocortical adenomas from three patients with Cushing's syndrome. The F content was significantly higher in adrenocortical adenomas than in normal adrenal glands, while the DHEA level was similar to that in normal adrenal glands. The adrenal adenomas showed a markedly higher level of P-450c17 mRNA, and a slightly but not significantly increased level of P-450c21 mRNA, compared with normal adrenal glands. The expression of P-450scc and P-450c11 mRNA in the adenomas was similar to that in normal adrenal glands. These results suggest that the overproduction of cortisol in adrenocortical adenomas associated with Cushing's syndrome results from an increased expression of P-450c17 and P-450c21 mRNA.
引用
收藏
页码：83 / 89
页数：7
相关论文
共 35 条
[1]   CUSHINGS-SYNDROME - PROBLEMS IN DIAGNOSIS [J].
ARON, DC ;
TYRRELL, JB ;
FITZGERALD, PA ;
FINDLING, JW ;
FORSHAM, PH .
MEDICINE, 1981, 60 (01) :25-35
[2]   IN VIVO AND IN VITRO STUDIES OF ADRENAL SECRETIONS IN CUSHINGS SYNDROME AND PRIMARY ALDOSTERONISM [J].
BIGLIERI, EG ;
SLATON, PE ;
HANE, S ;
FORSHAM, PH .
JOURNAL OF CLINICAL INVESTIGATION, 1963, 42 (04) :516-&
[3]   CHARACTERIZATION OF COMPLEMENTARY DEOXYRIBONUCLEIC-ACID FOR HUMAN ADRENOCORTICAL 17-ALPHA-HYDROXYLASE - A PROBE FOR ANALYSIS OF 17-ALPHA-HYDROXYLASE DEFICIENCY [J].
BRADSHAW, KD ;
WATERMAN, MR ;
COUCH, RT ;
SIMPSON, ER ;
ZUBER, MX .
MOLECULAR ENDOCRINOLOGY, 1987, 1 (05) :348-354
[4]  
CARPENTER PC, 1988, CLIN N AM, V17, P445
[5]   RAPID RADIOIMMUNOASSAY FOR DETERMINATION OF DEHYDROEPIANDROSTERONE SULFATE IN HUMAN PLASMA [J].
CATTANEO, S ;
FORTI, G ;
FIORELLI, G ;
BARBIERI, U ;
SERIO, M .
CLINICAL ENDOCRINOLOGY, 1975, 4 (05) :505-512
[6]   ISOLATION OF BIOLOGICALLY-ACTIVE RIBONUCLEIC-ACID FROM SOURCES ENRICHED IN RIBONUCLEASE [J].
CHIRGWIN, JM ;
PRZYBYLA, AE ;
MACDONALD, RJ ;
RUTTER, WJ .
BIOCHEMISTRY, 1979, 18 (24) :5294-5299
[7]   STEROID-BIOSYNTHESIS IN HUMAN ADRENAL-TUMORS [J].
DAGATA, R ;
MALOZOWSKI, S ;
BARKAN, A ;
CASSORLA, F ;
LORIAUX, D .
HORMONE AND METABOLIC RESEARCH, 1987, 19 (08) :386-388
[8]   MALE PSEUDOHERMAPHRODITISM CONSISTENT WITH 17,20-DESMOLASE DEFICIENCY [J].
GOEBELSMANN, U ;
ZACHMANN, M ;
DAVAJAN, V ;
ISRAEL, R ;
MESTMAN, JH ;
MISHELL, DR .
GYNECOLOGIC INVESTIGATION, 1976, 7 (03) :138-156
[9]   HYPERTENSION AND ALDOSTERONE OVERPRODUCTION WITHOUT RENIN SUPPRESSION IN CUSHINGS-SYNDROME FROM AN ADRENAL ADENOMA [J].
GUTHRIE, GP ;
KOTCHEN, TA .
AMERICAN JOURNAL OF MEDICINE, 1979, 67 (03) :524-528
[10]   COMPLETE NUCLEOTIDE-SEQUENCE OF 2 STEROID 21-HYDROXYLASE GENES TANDEMLY ARRANGED IN HUMAN-CHROMOSOME - A PSEUDOGENE AND A GENUINE GENE [J].
HIGASHI, Y ;
YOSHIOKA, H ;
YAMANE, M ;
GOTOH, O ;
FUJIIKURIYAMA, Y .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1986, 83 (09) :2841-2845
1234