TUNICAMYCIN INHIBITS THE INITIATION OF DNA-SYNTHESIS STIMULATED BY PROSTAGLANDIN-F2-ALPHA IN SWISS MOUSE 3T3 CELLS

被引：5

作者：

ESTEVEZ, AG ^{[1
]}

CALAMANTE, G ^{[1
]}

GOIN, M ^{[1
]}

DEASUA, LJ ^{[1
]}

机构：

[1] INST INVEST INGN GENET & BIOL MOLEC,OBLIGADO 2490,RA-1428 BUENOS AIRES,ARGENTINA

来源：

FEBS LETTERS | 1991年 / 290卷 / 1-2期

关键词：

SWISS; 3T3; CELL; PROSTAGLANDIN-F2-ALPHA; TUNICAMYCIN; DNA SYNTHESIS; LAG PHASE;

D O I：

10.1016/0014-5793(91)81269-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Tunicamycin, an inhibitor of the asparagine-linked protein N-glycosylation, blocks the initiation of DNA synthesis in Swiss 3T3 cells stimulated by prostaglandin F2-alpha alone or with insulin. This effect is exerted only when tunicamycin is added from 0 to 8 h after stimulation and it decreases the rate of entry into S phase. Blocking of labeled sugar incorporation to proteins occurs regardless of the time of PGF2-alpha stimulation. In contrast tunicamicin does not inhibit protein synthesis. These results suggest that N-glycoprotein synthesis early during the prereplicative phase is an important even controlling the mitogenic action of PGF2-alpha.

引用

页码：239 / 242

页数：4

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