EFFECT OF MEDIUM OSMOLARITY ON HYBRIDOMA GROWTH, METABOLISM, AND ANTIBODY-PRODUCTION

被引：147
作者：
OZTURK, SS [1 ]
PALSSON, BO [1 ]
机构：
[1] UNIV MICHIGAN,DEPT CHEM ENGN,ANN ARBOR,MI 48109
来源：
BIOTECHNOLOGY AND BIOENGINEERING | 1991年 / 37卷 / 10期
关键词：
Hybridoma; -; Osmolarity;
D O I：
10.1002/bit.260371015
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Osmolarity is an important process variable during the cultivation of mammalian cells in vitro. Cell culture medium is designed to have osmolarity in the range of 260 and 320 milliosmoles (mOsm), basically to mimic the osmolarity of serum at 290 mOsm/kg. Fragmented information on the response of hybridoma cells to elevated osmolarity is available. A decrease in cell growth and an increase in antibody production were reported at high osmolarities.4,7 Influence of osmolarity on antibody productivity seems to be cell line dependent.12 Here we report the effects of osmolarity on hybridoma cell growth, metabolism, and antibody synthesis. The osmolarity of the medium was altered by the addition of both ionic and non-ionic substances.
引用
收藏
页码：989 / 993
页数：5
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