A SENSITIVE METHOD OF SCREENING FOR DOMINANT T-CELL CLONES BY AMPLIFICATION OF T-CELL GAMMA-GENE REARRANGEMENTS WITH THE POLYMERASE CHAIN-REACTION

被引：40

作者：

GOUDIE, RB ^{[1
]}

KARIM, SN ^{[1
]}

MILLS, K ^{[1
]}

ALCORN, M ^{[1
]}

LEE, FD ^{[1
]}

机构：

[1] UNIV GLASGOW,ROYAL INFIRM,LEUKAEMIA RES FUND LABS,GLASGOW G4 0SF,SCOTLAND

来源：

JOURNAL OF PATHOLOGY | 1990年 / 162卷 / 03期

基金：

英国惠康基金;

关键词：

polymerase chain reaction; T cell lymphoma; T‐cell rearranging gamma genes; T‐cell receptor gene rearrangement; T‐lymphocyte clone;

D O I：

10.1002/path.1711620304

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A sensitive method of screening for dominant T cell clones in small samples of DNA has been developed using the polymerase chain reaction to amplify and identify T cell gamma receptor gene rearrangements. It can detect such rearrangements in nanogram quantities of DNA from cultured T cell clones, even in the presence of 20–100 parts of polyclonal lymph node DNA, and works with DNA extracted from paraffin sections of cloned T cells which have been fixed in formalin. Presumptive clonal reactions have been obtained in preliminary tests on 8 of 10 unfixed T cell lymphomas but in 0 of 10 reactive lymph nodes. Copyright © 1990 John Wiley & Sons, Ltd.

引用

页码：191 / 196

页数：6

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