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LYMPHOCYTE FIBROBLAST ADHESION - A USEFUL MODEL FOR ANALYSIS OF THE INTERACTION OF THE LEUKOCYTE INTEGRIN LFA-1 WITH ICAM-1
被引：17
|作者：
CABANAS, C
HOGG, N
机构：
[1] Macrophage Laboratory, Imperial Cancer Research Fund, London, WC2A 3PX
来源：
FEBS LETTERS
|
1991年
/
292卷
/
1-2期
关键词：
LFA-1;
ACTIVATION;
ICAM-1;
LEUKOCYTE ADHESION;
INTERCELLULAR ADHESION;
D O I：
10.1016/0014-5793(91)80885-7
中图分类号：
Q5 [生物化学];
Q7 [分子生物学];
学科分类号：
071010 ;
081704 ;
摘要：
The adhesion of human T lymphoblasts to ICAM-1-expressing normal dermal fibroblasts has been assessed as a sensitive model system for the analysis of the interaction of the leucocyte integrin LFA-1 with its counter-receptor ICAM-1. Using this model system, the effects of factors known to regulate the activity of LFA-1 have been quantitated: temperature; concentration of divalent cations; and exposure to phorbol esters. We show here that under the appropriate assay conditions, this model system represents a useful and simple alternative to the detection of leucocyte binding to purified ICAM-1 and also has the additional advantage of permitting more sensitive quantification than is possible using the homotypic adhesion assay.
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页码：284 / 288
页数：5
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