TRANSCRIPTIONAL REGULATION OF A PATATIN-1 GENE IN POTATO

被引:118
作者
JEFFERSON, R [1 ]
GOLDSBROUGH, A [1 ]
BEVAN, M [1 ]
机构
[1] INST PLANT SCI RES,CAMBRIDGE LAB,COLNEY LANE,NORWICH NR4 74J,ENGLAND
关键词
β-glucuronidase; patatin; sucrose; transgenic potatoes; tuber formation;
D O I
10.1007/BF00019396
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Patatin is an abundant glycoprotein in the tubers of potato plants that has a lipid acyl hydrolase activity. Fusions of the promoter of patatin genes that are highly expressed in tubers with the reporter gene encoding β-glucuronidase (GUS) have shown that patatin transcription has a high degree of tuber specificity. Patatin transcription was also inducible in other organs of transgenic potato by growth on high concentrations of sucrose. Experiments were conducted to define regions of the patatin promoter that confered tuber specific expression and sucrose inducibility. Sequences between -40 and -400 bp and between -400 and -957 bp of the transcriptional start site were able to confer tuber-specific expression on a heterologous truncated promoter. The cell specificity of GUS transcription in the transformants indicated that organ specificity was possibly determined by source-sink relationships of sucrose, or a metabolite of sucrose, in the whole plant. © 1989 Kluwer Academic Publishers.
引用
收藏
页码:995 / 1006
页数:12
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