NET ATP SYNTHESIS IN H-+-ATPASE MACROLIPOSOMES BY AN EXTERNAL ELECTRIC-FIELD

被引：52

作者：

ROGNER, M ^{[1
]}

OHNO, K ^{[1
]}

HAMAMOTO, T ^{[1
]}

SONE, N ^{[1
]}

KAGAWA, Y ^{[1
]}

机构：

[1] JICHI MED SCH,DEPT BIOCHEM & PHYS,MINAMIKAWACHI,TOCHIGI 32904,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1979年 / 91卷 / 01期

关键词：

D O I：

10.1016/0006-291X(79)90627-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Application of electric pulses (1000 V/cm, 20 m sec duration) to macroliposomes containing pure stable H+-ATPase (F0·F1) resulted in synthesis of ATP. Microliposomes containing F0·F1 showed very little ATP synthesis under the same conditions. The amount of ATP synthesized was increased by increasing the number of electric pulses applied and decreased by addition of either an uncoupler or an energy transfer inhibitor. © 1979.

引用

页码：362 / 367

页数：6

共 8 条

[1] RECONSTITUTION OF ENERGY TRANSFORMER, GATE AND CHANNEL SUBUNIT REASSEMBLY, CRYSTALLINE ATPASE AND ATP SYNTHESIS [J].

KAGAWA, Y .

BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 505 (01) :45-93

[2] RECONSTITUTION OF OXIDATIVE-PHOSPHORYLATION [J].

KAGAWA, Y .

BIOCHIMICA ET BIOPHYSICA ACTA, 1972, 265 (03) :297-+

[3]

Kagawa Y, 1979, Methods Enzymol, V55, P364

[4]

Kagawa Y, 1979, Methods Enzymol, V55, P781

[5] VECTORIAL CHEMISTRY AND MOLECULAR MECHANICS OF CHEMIOSMOTIC COUPLING - POWER TRANSMISSION BY PROTICITY [J].

MITCHELL, P .

BIOCHEMICAL SOCIETY TRANSACTIONS, 1976, 4 (03) :399-430

[6]

SONE N, 1977, J BIOL CHEM, V252, P2956

[7] ENERGY-CONVERSION IN THE FUNCTIONAL MEMBRANE OF PHOTOSYNTHESIS - ANALYSIS BY LIGHT-PULSE AND ELECTRIC PULSE METHODS - CENTRAL ROLE OF THE ELECTRIC-FIELD [J].

WITT, HT .

BIOCHIMICA ET BIOPHYSICA ACTA, 1979, 505 (3-4) :355-427

[8] MEMBRANE-BOUND ATP SYNTHESIS GENERATED BY AN EXTERNAL ELECTRICAL-FIELD [J].

WITT, HT ;

SCHLODDER, E ;

GRABER, P .

FEBS LETTERS, 1976, 69 (01) :272-276

← 1 →