EXPRESSION OF 2 FORMS OF PROLACTIN RECEPTOR IN RAT OVARY AND LIVER

The screening of a size-selected cDNA library from the ovary revealed the existence of a second form of PRL receptor in the rat. The polypeptide sequence deduced from cDNAs has a much longer cytoplasmic domain (357 amino acids) than the form previously identified in the liver (57 amino acids). Nucleotide sequence analysis and comparison with rabbit, mouse, and human PRL receptor cDNAs suggests that the two forms of rat PRL receptor result from alternative splicing of a primary transcript. Complementary DNAs encoding the long form of the receptor were also found in a library prepared from estradiol-treated rat liver, although they represent a minor fraction of total PRL receptor cDNAs obtained from this tissue. DNA polymerase chain reaction amplification of cDNA confirmed the presence of the two receptor forms in both the ovary and liver. Northern analysis, using probes that specifically hybridize with either form of mRNA, indicates a major transcript of 1.8 kilobases (kb) in estradiol-treated liver, which encodes the receptor with a short cytoplasmic domain, while the long form of the receptor is encoded by mRNAs of 2.5 and 3 kb. In the ovary, a complex pattern of hybridization to multiple mRNAs (1.8-5.5 kb) is obtained with the probe specific tc the long form, and essentially only a 5.5-kb mRNA is obtained with the probe specific to the short form, The predicted size of the mature form of the long PRL receptor (PRL-R2) is 591 amino acid residues The cytoplasmic domain of this receptor shows strong overall sequence similarity with the corresponding residues of the rabbit (67%) and humar (66%) PRL receptors and four regions of localized identity with the rabbit and human GH receptor. © 1990 by The Endocrine Society.