DETERMINATION OF CYTOKININS BY MASS-SPECTROMETRY BASED ON STABLE ISOTOPE DILUTION

被引：37

作者：

HASHIZUME, T

SUGIYAMA, T

IMURA, M

CORY, HT

SCOTT, MF

MCCLOSKEY, JA

机构：

[1] UNIV UTAH,DEPT MED CHEM,SALT LAKE CITY,UT 84112

[2] TOKYO UNIV AGR & TECHNOL,BIOORGAN CHEM LAB,FUCHU,TOKYO 183,JAPAN

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 92卷 / 01期

关键词：

D O I：

10.1016/0003-2697(79)90631-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for quantitative determination of individual cytokinin species has been developed, based on gas chromatography-mass spectrometry and selected ion monitoring. Deuterated internal standards were prepared for analysis of N6-isopentenyladenosine, N6-isopentenyl-2-methylthioadenosine, zeatin riboside, and 2-methylthiozeatin riboside and were tested over the range of 1 to 20 ng of endogenous cytokinin per injection, relative to 100 ng of labeled standard. An isolation procedure for extracts of cabbage hearts as a model plant source has been developed that gives maximum recovery and minimum interference for gas chromatographic-mass spectrometric measurements. The present method differs from the commonly used bioassay by its selectivity for individual cytokinin components and shortened analysis time, including extractions, of 3 days vs several weeks. © 1979.

引用

页码：111 / 122

页数：12

共 42 条

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