EXTENDING THE RANGE OF APPLICATION OF THE BIURET REACTION - QUANTITATIVE-DETERMINATION OF INSOLUBLE PROTEINS

被引：9
作者：
GOSHEV, I
NEDKOV, P
机构：
[1] Institute of Organic Chemistry, Bulgarian Academy of Sciences, Sofia
来源：
ANALYTICAL BIOCHEMISTRY | 1979年 / 95卷 / 02期
关键词：
D O I：
10.1016/0003-2697(79)90736-X
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A method for quantitative determination of insoluble proteins by means of the biuret reaction is proposed. The sample is oxidized with excess H2O2, dried, and subsequently dissolved in 0.05% sodium dodecyl sulfate-1 n NaOH at 100°C. The clear solution obtained is treated with biuret reagent and the absorbance of the chromogen is measured at 35-40°C. The method is applied successfully to collagen from bovine Achilles tendon, keratin of sheep's wool, and defatted sheepskin. Biuret chromogen absorbance of H2O2-oxidized human serum albumin is 10 ± 0.5% lower than that obtained with the native protein. © 1979.
引用
收藏
页码：340 / 343
页数：4
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