DETECTION OF AKR MULV-SPECIFIC RNA IN AKR MOUSE CELLS BY INSITU HYBRIDIZATION

被引：30

作者：

GODARD, C ^{[1
]}

JONES, KW ^{[1
]}

机构：

[1] INST ANIM GENET,EDINBURGH EH9 3JN,MIDLOTHIAN,SCOTLAND

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 6卷 / 08期

关键词：

D O I：

10.1093/nar/6.8.2849

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Conditions for the detection of complex RNA sequences by in situ hybridization have been investigated by using a single-stranded 3H-cDNA probe complementary to the AKR MuLV genome and in vitro cultured AKR mouse cells which spontaneously produce AKR MuLV. It is shown that fixation with glutaraldehyde at low concentration allows cellular RNA to be sufficiently well retained during the annealing process and that stringent conditions in situ can be maintained by means of formamide. Some conditions which promote atypical and non-specific binding of the probe have been identified. © 1979 Information Retrieval Limited.

引用

页码：2849 / 2861

页数：13

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