DIVERSITY OF CELLULOLYTIC BACTERIA IN LANDFILL

被引：32

作者：

WESTLAKE, K

ARCHER, DB

BOONE, DR

机构：

[1] NORWICH RES PK,INST FOOD RES,DEPT GENET & MICROBIOL,NORWICH,NORFOLK,ENGLAND

[2] OREGON GRAD INST SCI & TECHNOL,DEPT ENVIRONM SCI & ENGN,PORTLAND,OR

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1995年 / 79卷 / 01期

关键词：

D O I：

10.1111/j.1365-2672.1995.tb03126.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Nine of 37 cellulolytic bacterial isolates obtained from landfill waste could be easily differentiated on the basis of gross morphological characteristics. Four isolates were selected for further characterization and on the basis of initial results appear to be previously unidentified cellulolytic species of bacteria. An aerotolerant anaerobic, cellulolytic Clostridium and three obligately anaerobic cellulolytic Eubacterium isolates are described. The Clostridium has an unusually high pH optimum for growth of 7.7. The optimum temperature for growth is 50 degrees C. The pH growth optimum of each of the Eubacterium isolates is around pH 7.0 while temperature optima are 37 degrees, 45 degrees and 50 degrees C for LFI, LF4 and LF5 respectively. Most isolates had growth optima in the thermophilic range. The ease with which apparently previously unidentified species could be isolated is a reflection of the unique and highly variable, heterogeneous environment within landfill waste.

引用

页码：73 / 78

页数：6

共 19 条

[1]

Bagnara C., Toci R., Gaudin C., Belaich J.P., Isolation and characterization of a cellulolytic microorganism Cellulomonas fermentans sp. nov, International Journal of Systematic Bacteriology, 35, pp. 502-507, (1985)

[2]

Barlaz M.A., Ham R.K., Schaefer D.M., Massbalance analysis of anaerobically decomposed refuse, Journal of Environmental Engineering, 115, pp. 1088-1102, (1989)

[3]

Boone D.R., Johnson R.L., Liu Y., Diffusion of the interspecies electron carriers H<sub>2</sub> and formate in methanogenic ecosystems and its implications in the measurement of Km for H<sub>2</sub> or formate uptake, Appl Environ Microbiol, 55, pp. 1735-1741, (1989)

[4]

Bryant M.P., Burkey L.A., Cultural methods and some characteristics of some of the more numerous groups of bacteria in the bovine rumen, Journal of Dairy Science, 36, pp. 205-217, (1953)

[5]

Campbell D.J.V., Croft B., pp. 281-303, (1991)

[6]

Cecchi F., Pavan P., Alvarez J.M., Bassetti A., Cozzolino C., Anaerobic digestion of municipal solid waste: Thermophilic vs. mesophilic performance at high solids, Waste Management Research, 9, pp. 305-314, (1991)

[7]

The sustainable landfill: A feasibility study to assess the potential of developing a bioreactor cell rotational landfill, ETSU Report B/B3/00242/REP., (1993)

[8]

Fielding E.R., Archer D.B., Conway de Macario E., Macario A.J.L., Isolation and characterisation of methanogenic bacteria from landfills, Applied and Environmental Microbiology, 54, pp. 835-836, (1988)

[9]

Gendebien A., Pauwels M., Constant M., Ledrut-Damanet M.-J., Nyns E.-J., Willumsen H.C., Butson J., Fabry R., Ferrero G.-L., (1992)

[10]

Anaerobic Laboratory Manual, (1977)

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