IN-VITRO ISOLATION AND CULTIVATION OF A BABESIA FROM AN AMERICAN WOODLAND CARIBOU (RANGIFER TARANDUS CARIBOU)

被引：22
|
作者：
HOLMAN, PJ
PETRINI, K
RHYAN, J
WAGNER, GG
机构：
[1] MINNESOTA ZOOL GARDENS,APPLE VALLEY,MN 55124
[2] USDA,VET SERV,NATL VET SERV LABS,AMES,IA 50010
来源：
JOURNAL OF WILDLIFE DISEASES | 1994年 / 30卷 / 02期
关键词：
BABESIA SP; CARIBOU; RANGIFER TARANDUS CARIBOU; BABESIOSIS; ZOO;
D O I：
10.7589/0090-3558-30.2.195
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
A Babesia species isolated from a captive caribou (Rangifer tarandus caribou) with clinical signs of babesiosis and a circulating parasitemia was cultured in vitro. Normal adult caribou erythrocytes supported the growth of the Babesia sp., as did erythrocytes from white-tailed deer (Odocoileus virginianus). Two basal media (M-199 and RPMI-1640) and a defined medium (HL-1) each supplemented with adult bovine serum were compared. The most favorable growth of the parasite occurred in HL-1 medium with 20% adult bovine serum. The morphology of this Babesia sp. isolate shared some characteristics with B. odocoilei and B. divergens.
引用
收藏
页码：195 / 200
页数：6
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