Automated extraction of DNA and PCR setup using a Tecan Freedom EVO (R) liquid handler

被引：6
|
作者：
Stangegaard, Michael [1 ]
Froslev, Tobias G. [1 ]
Frank-Hansen, Rune [1 ]
Laursen, Susan S. [1 ]
Jorgensen, Mads [1 ]
Hansen, Anders J. [1 ]
Morling, Niels [1 ]
机构：
[1] Univ Copenhagen, Fac Hlth Sci, Dept Forens Med, Sect Forens Genet, Copenhagen, Denmark
来源：
FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES | 2009年 / 2卷 / 01期
关键词：
Laboratory automation; Nucleic acid extraction; Magnetic beads; Reference samples;
D O I：
10.1016/j.fsigss.2009.07.002
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
We have implemented and validated automated methods for DNA extraction and PCR setup developed for a Tecan Freedom EVO (R) liquid handler mounted with a Te-MagS (TM) magnetic separation device. The DNA was extracted using the Qiagen MagAttract (R) DNA Mini M48 kit. The DNA was amplified using AmpFlSTR (R) Identifiler (R), Y-filer (R) (Applied Biosystems), GenePrint (R) FFFL and PowerPlex (R) Y (Promega). The methods were validated for fresh whole blood and blood from deceased according to EN/ISO 17025. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码：74 / 76
页数：3
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