SEPARATION OF FLUORESCENT OLIGOSACCHARIDE DERIVATIVES BY MICROCOLUMN TECHNIQUES BASED ON ELECTROPHORESIS AND LIQUID-CHROMATOGRAPHY

被引：58

作者：

LIU, JP ^{[1
]}

SHIROTA, O ^{[1
]}

NOVOTNY, M ^{[1
]}

机构：

[1] INDIANA UNIV,DEPT CHEM,BLOOMINGTON,IN 47405

来源：

JOURNAL OF CHROMATOGRAPHY | 1991年 / 559卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(91)80073-P

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Various aldose oligosaccharides can be quantitatively derivatized into primary amines for subsequent reaction with fluorogenic reagents, such as 3-(4-carboxybenzoyl)-2-quinolinecarboxaldehyde or 3-benzoyl-2-naphthaldehyde. Capillary electrophoresis (CE) and microcolumn liquid chromatography (LC), coupled with laser-induced fluorescence detection, were evaluated as a means of separating complex oligosaccharide mixtures. Whereas microcolumn LC and open-tubular CE appear confined in their utility to relatively small oligosaccharides, unprecedented results were obtained with polyacrylamide gel-filled capillaries on hydrolyzed malto-oligosaccharides and enzymatically degraded samples of chondroitin sulfate and hyaluronic acid.

引用

页码：223 / 235

页数：13

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