DETERMINATION OF THE CURCUMINOID PIGMENTS IN TURMERIC (CURCUMA-DOMESTICA VAL) BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：60

作者：

TAYLOR, SJ

MCDOWELL, IJ

机构：

[1] Natural Resources Institute, Chatham Maritime, ME4 4TB, Chatham, Central Avenue

来源：

CHROMATOGRAPHIA | 1992年 / 34卷 / 1-2期

关键词：

COLUMN LIQUID CHROMATOGRAPHY; CURCUMINOID PIGMENTS; TURMERIC; CURCUMA-DOMESTICA VAL;

D O I：

10.1007/BF02290463

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A rapid, simple and reproducible reversed-phase high-performance liquid chromatographic method was developed for the quantitative determination of curcumin, demethoxycurcumin and bis-demethoxycurcumin in ethanolic extracts of turmeric. The pigments were separated on a styrene-divinylbenzene copolymer column (Hamilton PRP-1), using an acetonitrile-water (55:45, v/v) mobile phase. The pigments were monitored with a diode-array detector at 425 nm. The limit of detection was 10.2 ng curcumin, 11.1 ng demethoxycurcumin and 6.2 ng bisdemethoxycurcumin. Comparison of HPLC and spectrophotometric results for the determination of the total curcuminoid content for a number of turmeric samples, reveal that the spectrophotometric method invariably yielded higher results, indicating an overestimation of curcuminoids.

引用

页码：73 / 77

页数：5

共 13 条

[1] COMPARATIVE-STUDY OF THE REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF BLACK TEA LIQUORS WITH SPECIAL REFERENCE TO THE THEARUBIGINS
BAILEY, RG
NURSTEN, HE
MCDOWELL, I
[J]. JOURNAL OF CHROMATOGRAPHY, 1991, 542 (01): : 115 - 128
[2] THIN-LAYER CHROMATOGRAPHIC DETERMINATION OF CURCUMINE (TURMERIC) IN SPICES
JANSSEN, A
GOLE, T
[J]. CHROMATOGRAPHIA, 1984, 18 (10) : 546 - 549
[3] JENTZSCH K, 1970, SCI PHARM VIENNA, V38, P50
[4] Krishnamurthy M. N., 1975, Journal of Food Science and Technology, India, V12, P12
[5] SOME OBSERVATION ON CURCUMINOIDS FROM ZINGIBERACEAE PLANTS
KUROYANA.M
NATORI, S
[J]. YAKUGAKU ZASSHI, 1970, 90 (11): : 1467 - &
[6] Purseglove J. W., 1981, TROPICAL AGR SERIES, V2, P544
[7] EXPERIMENTS IN BIOSYNTHESIS OF CURCUMIN
ROUGHLEY, PJ
WHITING, DA
[J]. JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1973, (20): : 2379 - 2388
[8] SALTZER UJ, 1975, FLAVOURS, V206, P253
[9] COMPARISON OF DETECTORS FOR THE DETERMINATION OF CURCUMIN IN TURMERIC BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
SMITH, RM
WITOWSKA, BA
[J]. ANALYST, 1984, 109 : 259 - 261
[10] A CHROMATOGRAPHIC STUDY OF THE CURCUMINOIDS IN CURCUMA-LONGA, L
SRINIVASAN, KR
[J]. JOURNAL OF PHARMACY AND PHARMACOLOGY, 1953, 5 (07) : 448 - 457

← 1 2 →