OPTIMIZATION OF FAT-SOLUBLE VITAMIN SEPARATION BY SUPERCRITICAL-FLUID CHROMATOGRAPHY

被引：10

作者：

IBANEZ, E ^{[1
]}

TABERA, J ^{[1
]}

REGLERO, G ^{[1
]}

HERRAIZ, M ^{[1
]}

机构：

[1] CSIC,INST FERMENTAC IND,E-28006 MADRID,SPAIN

来源：

CHROMATOGRAPHIA | 1995年 / 40卷 / 7-8期

关键词：

SUPERCRITICAL FLUID CHROMATOGRAPHY; MICROPACKED COLUMNS; FAT-SOLUBLE VITAMINS; EXPERIMENTAL DESIGN;

D O I：

10.1007/BF02269911

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fat-soluble vitamin separation, achievable using a micropacked SFC column loaded with Cw 20M, is optimized using a rotatable central composite experimental design. Two chromatographic response functions, based on relative retention and the number of peaks resolved are proposed. Temperature and pressure gradients giving the best response in the experimental region are obtained and the effect of their variation on separation is evaluated. Relative standard deviations resulting from both absolute and normalized peak areas are also given.

引用

页码：448 / 452

页数：5

共 23 条

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