Test of the rapid PCR method using AmpFlSTR Identifiler kit

被引：10

作者：

Choung, Chong Min ^{[1
]}

Lee, Dong Sub ^{[1
]}

Park, Ki Won ^{[1
]}

Han, Myun Soo ^{[1
]}

机构：

[1] Forens DNA Ctr, Natl Forens Serv, 139 Jiyangno, Seoul 158707, South Korea

来源：

FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES | 2011年 / 3卷 / 01期

关键词：

DNA typing; Identifler; Rapid PCR;

D O I：

10.1016/j.fsigss.2011.09.099

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Current forensic DNA typing is conducted in approximately 8-9 h. Whole steps included DNA extraction step, Quantification, PCR amplification step, electrophoresis process through capillary separation with fluorescence detection, data analysis and DNA profile interpretation. Among them, we have tested rapid PCR method of AmpFlSTR Identifler PCR to reduce running time. We have altered several PCR conditions of ordinary AmpFlSTR Identifler PCR method and used 9947A control DNA to cut the time for the PCR reaction. In the results of this study, the critical step of the PCR reaction was the annealing step and also we reduced PCR running times by 1/3 to 2/3 (approximately 60-90 min) with complete concordance of STR allele calls using standard reference material 9947A. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

引用

页码：E475 / E476

页数：2