PHYLOGENETIC-RELATIONSHIPS OF ANNUAL AND PERENNIAL WILD-RICE - PROBING BY DIRECT DNA SEQUENCING

被引：39

作者：

BARBIER, P

MORISHIMA, H

ISHIHAMA, A

机构：

[1] NATL INST GENET,DEPT MOLEC GENET,MISHIMA,SHIZUOKA 411,JAPAN

[2] NATL INST GENET,DEPT AGR GENET,MISHIMA,SHIZUOKA 411,JAPAN

来源：

THEORETICAL AND APPLIED GENETICS | 1991年 / 81卷 / 05期

关键词：

ASIAN WILD RICE; PHYTOCHROME INTRON; POLYMERASE CHAIN REACTION (PCR); DIRECT DNA SEQUENCING; INTRASPECIFIC DNA POLYMORPHISM;

D O I：

10.1007/BF00226739

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

The phylogenetic relationships between Asian wild rice strains were analyzed by direct sequencing of PCR-amplified DNA fragments. The sequence of three introns located in the phytochrome gene was determined for eight strains of the Asian wild rice, Oryza rufipogon, and one strain of the related African species, Oryza longistaminata. The number of nucleotide substitutions per site between various strains within a single species, O. rufipogon, ranged between 0.0017 and 0.0050, while those between two related species, O. rufipogon and O. longistaminata, were 0.043-0.049 (23-26 within 532 bp). Taken together with the sequence differences of the 10kDa prolamin gene, a model is proposed for the phylogenetic relationships and evolutionary history of annuals and perennials within O. rufipogon.

引用

页码：693 / 702

页数：10

共 37 条

[1]

[Anonymous], 1987, MOL EVOLUTIONARY GEN, DOI DOI 10.7312/NEI-92038

[2]

AQUADRO CF, 1988, GENETICS, V119, P875

[3] GENETIC-VARIATION AND ECOTYPIC DIFFERENTIATION IN THE WILD RICE SPECIES ORYZA-RUFIPOGON .1. POPULATION DIFFERENTIATION IN LIFE-HISTORY TRAITS AND ISOZYMIC LOCI [J].