ENRICHMENT OF BIOLOGICALLY-ACTIVE U1 SMALL NUCLEAR RNAS BY ION-EXCHANGE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

被引：1
作者：
LEFF, V [1 ]
GAO, JP [1 ]
VEGA, LR [1 ]
HERRERA, RJ [1 ]
机构：
[1] FLORIDA INT UNIV,DEPT BIOL SCI,MIAMI,FL 33199
来源：
JOURNAL OF CHROMATOGRAPHY | 1991年 / 547卷 / 1-2期
关键词：
D O I：
10.1016/S0021-9673(01)88673-8
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The use of ion-exchange high-performance liquid chromatography in conjunction with preparative electrophoresis to facilitate the purification of biologically active snRNAs is described. Separation of total nuclear RNA from a Bombyx mori cell line was done with a Bio-Rad MA7 plasmid column in a HRLC 500 system. Individual fractions were subjected to electrophoresis through 14% polyacrylamide gels for identification. High levels of U1 RNA were confirmed by Northern analysis with a human U1 probe. Biological activity of RNAs from the column was demonstrated by their ability to incorporate P-32-AMP at the 3' end. lon-exchange chromatography provides a rapid, automated method for purifying large amounts of RNAs that can then be utilized in further studies.
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页码：462 / 467
页数：6
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