HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF MULTIVITAMIN PREPARATIONS USING A CHEMICALLY BONDED CYCLODEXTRIN STATIONARY PHASE

被引：18

作者：

ELGIZAWY, SM

AHMED, AN

ELRABBAT, NA

机构：

[1] College of Pharmacy, Dept. of Pharm. Chem, Assiut University, Assiut

来源：

ANALYTICAL LETTERS | 1991年 / 24卷 / 07期

关键词：

HPLC; MULTIVITAMINS; CYCLODEXTRIN STATIONARY PHASE; WATER-SOLUBLE VITAMINS; PHARMACEUTICAL PREPARATIONS; FOLIC-ACID; NIACINAMIDE;

D O I：

10.1080/00032719108052962

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high performance liquid chromatographic procedure for simultaneous determination of water-soluble vitamin preparations containing pyridoxine hydrochloride (Vit. B6, cyanocobalamin (Vit. B12), thiamine hydrochloride (Vit. B1) and folic acid using a cyclobond I column was developed. The four vitamins were assayed in multivitamin pharmaceutical formulations not containing minerals. Samples were chromatographed using pH 7 phosphate buffer: methanol (80:20 %) mobile-phase. The procedure is rapid and the total chromatographic time does not exceed 6.64 min. The method is accurate, precise and specific.

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页码：1173 / 1181

页数：9

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