CLONING OF CLOSTRIDIUM THERMOCELLUM ENDOGLUCANASE GENES IN ESCHERICHIA-COLI

被引：23
|
作者：
BUMAZKIN, BK
VELIKODVORSKAYA, GA
TUKA, K
MOGUTOV, MA
STRONGIN, AY
机构：
[1] ACAD SCI USSR,INST MOLEC GENET,KURCHATOV SQ,MOSCOW 123182,USSR
[2] ACAD SCI USSR,CENDISI BIOPROC,MOSCOW 121069,USSR
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 167卷 / 03期
关键词：
D O I：
10.1016/0006-291X(90)90630-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Six independent and distinct cel genes coding endoglucanases have been selected from C. thermocellum pUC19-based gene bank in E.coli TG1. E.coli-derived Cel-proteins possessing Mr from 39,000 to 61,000 are able to cleave lichenan, as well as xylan and carboxymethyl cellulose. Cel 7- and Cel 8-endoglucanases are characterized by cellobiohydrolase type substrate specificity, being able to cleave model fluorogenic aryldisaccharide substrate MU-G2. The clone pCU110 (cel 7) produces about 10-fold more endoglucanase activity than other clones. © 1990.
引用
收藏
页码：1057 / 1064
页数：8
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