IDENTIFICATION AND STRUCTURAL CHARACTERIZATION OF A MANNOSE-6-PHOSPHATE CONTAINING OLIGOMANNOSIDIC N-GLYCAN FROM HUMAN ERYTHROPOIETIN SECRETED BY RECOMBINANT BHK-21-CELLS

被引：33

作者：

NIMTZ, M ^{[1
]}

WRAY, V ^{[1
]}

RUDIGER, A ^{[1
]}

CONRADT, HS ^{[1
]}

机构：

[1] GESELL BIOTECHNOL FORSCH MBH,DEPT CELL BIOL & GENET,D-38124 BRAUNSCHWEIG,GERMANY

来源：

FEBS LETTERS | 1995年 / 365卷 / 2-3期

关键词：

RECOMBINANT HUMAN ERYTHROPOIETIN; BABY HAMSTER KIDNEY CELLS (BHK-21); PHOSPORYLATED OLIGOMANNOSIDIC GLYCAN; MANNOSE-6-PHOSPHATE; 1- AND 2-D NMR SPECTROSCOPY; LYSOSOMAL RECOGNITION SIGNAL;

D O I：

10.1016/0014-5793(95)00473-M

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A sialidase resistant mono-charged N-glycan was isolated from glycosylation site I (Asn-24) of recombinant human erythropoietin expressed from baby hamster kidney (BHK-21) cells and constituted approximately 2-4% of the oligosaccharide material at this glycosylation site. Mass spectrometry and both 1- and 2-dimensional NMR techniques revealed a high mannose type structure (Man(6)) with a phospho-diesterbridged N-acetylglucosamine as follows: [GRAPHICS] and FK-506 binding proteins [Rahfeld et al, (1994) FEES Lett, 352, 180-184]. Should Ptf1p display PPIase activity, it would be the first characterized, eukaryotic member of this putative family, which is essential for growth.

引用

页码：203 / 208

页数：6

共 3 条

[1] N-GLYCOSYLATION AND O-GLYCOSYLATION MUTEINS OF RECOMBINANT-HUMAN-ERYTHROPOIETIN SECRETED FROM BHK-21-CELLS
FIBI, MR
HERMENTIN, P
PAULY, JU
LAUFFER, L
ZETTLMEISSL, G
BLOOD, 1995, 85 (05) : 1229 - 1236
[2] Chemical Synthesis of a Bisphosphorylated Mannose-6-Phosphate N-Glycan and its Facile Monoconjugation with Human Carbonic Anhydrase II for in vivo Fluorescence Imaging
Liu, Yunpeng
Chan, Yan Mei
Wu, Jianhui
Chen, Chen
Benesi, Alan
Hu, Jing
Wang, Yanming
Chen, Gong
CHEMBIOCHEM, 2011, 12 (05) : 685 - 690
[3] CONSTRUCTION OF STABLE BHK-21-CELLS COEXPRESSING HUMAN SECRETORY GLYCOPROTEINS AND HUMAN GAL(BETA-1-4)GLCNAC-R ALPHA-2,6-SIALYLTRANSFERASE ALPHA-2,6-LINKED NEUAC IS PREFERENTIALLY ATTACHED TO THE GAL(BETA-1-4)GLCNAC(BETA-1-2)MAN(ALPHA-1-3)-BRANCH OF DIANTENNARY OLIGOSACCHARIDES FROM SECRETED RECOMBINANT BETA-TRACE PROTEIN
GRABENHORST, E
HOFFMANN, A
NIMTZ, M
ZETTLMEISSL, G
CONRADT, HS
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1995, 232 (03): : 718 - 725

← 1 →