DETERMINATION OF PANOMIFENE IN HUMAN PLASMA BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

An ion-pair HPLC method was developed for the determination of the antiestrogenic drug panomifene, (E)-1,2-diphenyl-1-{4-[2-(2-hydroxyethylamino)ethoxy]phenyl}-3,3,3-trifluoropropene, in human plasma. Tamoxifen, 20 ng in 1 ml of plasma, was used as an internal standard. The compounds were isolated from plasma by liquid-solid extraction. Fluorescence detection was achieved by on-line photochemical conversion of the compounds into highly fluorescent phenanthrene derivatives. The sensitivity of the method was 1 ng/ml. The within-day and between-day precision, linearity, extraction recovery and stability of panomifene in plasma and in deproteinized plasma were determined for validation of the method. The method is suitable for measuring plasma levels of panomifene and tamoxifen and for pharmacokinetic studies.