ELEVATED GENE-EXPRESSION OF ARGININOSUCCINATE SYNTHETASE IN PERIPHERAL LYMPHOCYTES FROM SYSTEMIC LUPUS-ERYTHEMATOSUS (SLE) PATIENTS

被引：8

作者：

OHNO, T ^{[1
]}

KIMURA, Y ^{[1
]}

SUGIMURA, K ^{[1
]}

SAGAWA, A ^{[1
]}

JHODO, S ^{[1
]}

AZUMA, I ^{[1
]}

机构：

[1] HOKKAIDO UNIV,INST IMMUNOL SCI,SAPPORO,HOKKAIDO 060,JAPAN

来源：

AUTOIMMUNITY | 1992年 / 13卷 / 02期

关键词：

SLE; ARGININOSUCCINATE SYNTHETASE; GENE EXPRESSION; POLYMERASE-CHAIN REACTION; L-ARGININE; UREA CYCLE;

D O I：

10.3109/08916939209001913

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Argininosuccinate synthetase (ASS) is a rate-limiting enzyme of urea cycle and functions primarily in the liver, whereas ASS activity is hardly detected in normal lymphocytes. In this study, we examined the level of ASS gene expression in peripheral blood lymphocytes (PBL) from human SLE patients by amplification of reverse-transcribed mRNA using the polymerase chain reaction. We have demonstrated that (a) approximately 40% of SLE patients exhibited 2.5 to 5 times higher expression of ASS gene in PBL than those of healthy PBL and (b) the elevation of ASS gene expression of PBL significantly correlates with the active pathogenesis of SLE patients according to the criteria of Japanese Ministry of Health and Welfare (p<0.001 by student's two-tailed t-test). Thus, it is suggested that ASS gene expression is a promising marker of hyperactivated lymphocytes uniquely generated in patients with systemic autoimmune disease.

引用

页码：127 / 132

页数：6

共 27 条

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