MICROTUBULE AFFINITY-CHROMATOGRAPHY - A NEW TECHNIQUE FOR ISOLATING MICROTUBULE-BINDING PROTEINS FROM RAT PANCREAS

被引：1

作者：

MICHALIK, L ^{[1
]}

VANIER, MT ^{[1
]}

LAUNAY, JF ^{[1
]}

机构：

[1] INSERM, U61, UNITE BIOL CELLULAIRE & PHYSIOPATHOL DIGEST, 3 AVE MOLIERE, F-67200 STRASBOURG, FRANCE

来源：

CELLULAR AND MOLECULAR BIOLOGY | 1991年 / 37卷 / 08期

关键词：

RAT; PANCREAS; MICROTUBULE-ASSOCIATED PROTEINS; TAU-PROTEIN;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed an affinity chromatography method for the isolation of microtubule-associated proteins (MAPs) from soluble cytoplasmic extracts of rat pancreas. Among the ten proteins which copurify with pancreas tubulin on a colchicine derivatives-affinity chromatography, three polypeptides of respectively 58,55 and 48 kDa strongly bind to the microtubule affinity column. To begin to characterize these proteins, we have generated polyclonal antibodies against tau polypeptides from brains of immature chicken or rat. As judged by immunoblots, the three polypeptides seem to be immunologically related to the tau proteins previously localized in brain.

引用

页码：805 / 811

页数：7

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