ABOUT SULFUR-CONTAINING GLYCOSIDES OF RAPE SEEDS - ANALYSIS BY THERMOSPRAY TECHNIQUE MASS-SPECTROMETRY

The HPLC is the method of choise for the estimation of glucosinolates occurring in rape seeds. But in comparison to GC a disadvantage is that the HPLC fractions can't be analyzed by MS directly. Such possibility is opened now with the thermospray technique. It allows to introduce the HPLC fractions immediately into the mass spectrometer system. Based on the first application of this technique to glucosinolates published by Mellon et al. (1987) the fundamentals of the thermospray technique/mass spectrometry are presented, the experimental details are explained and the characteristic ions of the thermospray mass spectra of 12 glucosinolates (desulfo derivatives) are discussed. The analytical performance of the method is examined also.