POLYAMINES AND CELLULAR ADENOSINE 3'-5'-CYCLIC MONOPHOSPHATE

被引：22

作者：

CLO, C ^{[1
]}

CALDARERA, CM ^{[1
]}

TANTINI, B ^{[1
]}

BENALAL, D ^{[1
]}

BACHRACH, U ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM,HADASSAH HOSP & MED SCH,DEPT MOLEC BIOL,JERUSALEM,ISRAEL

来源：

BIOCHEMICAL JOURNAL | 1979年 / 182卷 / 03期

关键词：

D O I：

10.1042/bj1820641a

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of polyamines on the cellular concentrations of cyclic AMP was studied. It was shown that 1 μm-spermene caused a decrease in cyclic AMP in chick-embryo heart cells, chick-embryo fibroblasts, neuroblastoma, glioma and neuroblastoma-glioma hybrid cells, grown in culture. A similar decrease was observed when polyamines were added to cells in the presence of a phosphodiesterase inhibitor or after stimulating the cells with various hormones. Noradrenaline was used in cultures of heart cells, prostaglandin E1 and adenosine for neuroblastoma and neuroblastoma-glioma hybrids, whereas isoproterenol was used for the stimulation of glioma cells. Polyamines at higher concentrations were either without effect or caused a slight increase in cyclic AMP. Spermidine (10 μm) also caused a decreased in cellular cyclic AMP, as did 0.1 μm-putrescine. It is suggested that the effect of polyamines on cellular cyclic AMP may be explained by the effect of these polycations on the activity of cellular phosphodiesterase.

引用

页码：641 / 649

页数：9

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