ASSAY OF COMPLEMENT ACTIVATION IN MOUSE SERUM BY ELISA

被引：2

作者：

MORRISON, LJA ^{[1
]}

BEHAN, PO ^{[1
]}

BEHAN, WMH ^{[1
]}

机构：

[1] WESTERN INFIRM & ASSOCIATED HOSP, DEPT PATHOL, GLASGOW G11 6NT, SCOTLAND

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 126卷 / 02期

关键词：

(Mouse); Complement activation; ELISA;

D O I：

10.1016/0022-1759(90)90150-T

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a simple, sensitive and reproducible ELISA assay for complement activation in mouse serum, based on measurement of the opsonization of purified, heat-aggregated mouse gamma-globulin bound to the solid phase. This assay measures mainly classical pathway activation, and we used it to show significant differences in complement activation in sera from mice of various autoimmune, immunodeficient and immunologically normal strains. This assay has the advantage over existing methods of requiring minimal volumes of serum, and of avoiding problems of species incompatibility. © 1990.

引用

页码：191 / 197

页数：7

共 24 条

[1] SPONTANEOUS MURINE LUPUS-LIKE SYNDROMES - CLINICAL AND IMMUNOPATHOLOGICAL MANIFESTATIONS IN SEVERAL STRAINS [J].

ANDREWS, BS ;

EISENBERG, RA ;

THEOFILOPOULOS, AN ;

IZUI, S ;

WILSON, CB ;

MCCONAHEY, PJ ;

MURPHY, ED ;

ROTHS, JB ;

DIXON, FJ .

JOURNAL OF EXPERIMENTAL MEDICINE, 1978, 148 (05) :1198-1215

[2] MICROASSAY FOR DETERMINATION OF HEMOLYTIC COMPLEMENT ACTIVITY IN MOUSE SERUM [J].

ANDREWS, BS ;

THEOFILOPOULOS, AN .

JOURNAL OF IMMUNOLOGICAL METHODS, 1978, 22 (3-4) :273-281

[3]

ATKINSON JP, 1980, CLIN IMMUNOLOGY, V1, P219

[4] ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) QUANTITATIVE ASSAY OF IMMUNOGLOBULIN-G [J].

ENGVALL, E ;

PERLMANN, P .

IMMUNOCHEMISTRY, 1971, 8 (09) :871-&

[5]

FESTING MFW, 1979, INBRED STRAINS BIOME, P226

[6]

GOLDMAN MB, 1976, J IMMUNOL, V117, P1584

[7]

HARRISON RA, 1986, HDB EXPT IMMUNOLOGY, V1

[8]

JOHNSON AR, 1975, IMMUNOLOGY, V28, P1067

[9]

KLAUS GGB, 1977, IMMUNOLOGY, V33, P31

[10] NEW BIOLOGICAL-ACTIVITY FOLLOWING INTRAVASCULAR ACTIVATION OF COMPLEMENT CASCADE [J].

MCCALL, CE ;

DECHATELET, LR ;

BROWN, D ;

LACHMANN, P .

NATURE, 1974, 249 (5460) :841-843

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