ISOCRATIC REVERSE-PHASE HPLC SEPARATION AND RIA USED IN THE ANALYSIS OF NEUROPEPTIDES IN BRAIN-TISSUE

被引：12
作者：
DECEBALLOS, ML
TAYLOR, MD
JENNER, P
机构：
[1] KINGS COLL LONDON,DIV BIOMED SCI,PHARMACOL GRP,PARKINSONS DIS SOC,EXPTL RES LABS,MANRESA RD,LONDON SW3 6LX,ENGLAND
[2] CSIC,CAJAL INST,DEPT NEUROPHARMACOL,E-28002 MADRID,SPAIN
来源：
NEUROPEPTIDES | 1991年 / 20卷 / 03期
关键词：
D O I：
10.1016/0143-4179(91)90132-3
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
A reverse-phase, high-performance liquid chromatographic (HPLC) method was employed to separate and characterise five neuropeptides from complex mixtures, with important advantages over methods employed earlier using combined HPLC-RIA studies. Peptides were separated using 0.5 M pyridine - 0.5 M formic acid buffer, pH 4, containing propan-l-ol 14% (met-enkephalin, leu-enkephalin, neurotensin) or 20% (CCK-8-S, substance P) at a flow rate of 1.0 ml/min. Isocratic conditions, and volatile solvents, resulted in a highly reproducible method, producing samples in a form designed for subsequent RIA. The application and importance of the procedure is demonstrated by comparison of the Measurements of apparent peptide levels in crude brain extracts with those of authentic peptides as determined after HPLC purification.
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页码：201 / 209
页数：9
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