SYSTEMATIC LIQUID-CHROMATOGRAPHIC SEPARATION OF POLYSACCHARIDES, OLIGOSACCHARIDES AND MONOSACCHARIDES

被引：19

作者：

NOEL, D ^{[1
]}

HANAI, T ^{[1
]}

DAMBOISE, M ^{[1
]}

机构：

[1] UNIV MONTREAL,DEPT CHEM,MONTREAL H3C 3V1,QUEBEC,CANADA

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1979年 / 2卷 / 09期

关键词：

D O I：

10.1080/01483917908060141

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

High speed separations of poly-, oligo-, and monosaccharides were achieved on Chromosorb LC 9, Toyo Soda Starch Gel TSKLS170P5, and Hitachi 3013N packings. Post column reaction system with tetrazolium blue was used to obtain a low detection limit for oligo-, and monosaccharides. Polysaccharides were analysed within 20 min by gel permeation chromatography over TSKLS170P5 gel. DP 30 oligosaccharide was eluted in 30 min on Chromosorb LC 9 using a gradient of acetonitrile/water. Finally monosaccharides were separated in 25 min on Hitachi 3013N macroporous anion exchang resin. Copyright © 1979 by Marcel Dekker, Inc.

引用

页码：1325 / 1336

页数：12

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