SYSTEMATIC LIQUID-CHROMATOGRAPHIC SEPARATION OF POLYSACCHARIDES, OLIGOSACCHARIDES AND MONOSACCHARIDES

被引：19
作者：
NOEL, D [1 ]
HANAI, T [1 ]
DAMBOISE, M [1 ]
机构：
[1] UNIV MONTREAL,DEPT CHEM,MONTREAL H3C 3V1,QUEBEC,CANADA
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY | 1979年 / 2卷 / 09期
关键词：
D O I：
10.1080/01483917908060141
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
High speed separations of poly-, oligo-, and monosaccharides were achieved on Chromosorb LC 9, Toyo Soda Starch Gel TSKLS170P5, and Hitachi 3013N packings. Post column reaction system with tetrazolium blue was used to obtain a low detection limit for oligo-, and monosaccharides. Polysaccharides were analysed within 20 min by gel permeation chromatography over TSKLS170P5 gel. DP 30 oligosaccharide was eluted in 30 min on Chromosorb LC 9 using a gradient of acetonitrile/water. Finally monosaccharides were separated in 25 min on Hitachi 3013N macroporous anion exchang resin. Copyright © 1979 by Marcel Dekker, Inc.
引用
收藏
页码：1325 / 1336
页数：12
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