IMMUNOCYTOCHEMICAL MAPPING OF NEURONAL PATHWAYS FROM BRAIN TO CORPORA CARDIACA CORPORA ALLATA IN THE COCKROACH DIPLOPTERA-PUNCTATA WITH ANTISERA AGAINST MET-ENKEPHALIN-ARG6-GLY7-LEU8

被引：11

作者：

DUVE, H ^{[1
]}

THORPE, A ^{[1
]}

TOBE, SS ^{[1
]}

机构：

[1] UNIV TORONTO,DEPT ZOOL,TORONTO M5S 1A1,ONTARIO,CANADA

来源：

CELL AND TISSUE RESEARCH | 1991年 / 263卷 / 02期

关键词：

MET-ENKEPHALIN-ARG6-GLY7-LEU8; IMMUNOCYTOCHEMISTRY; NEUROPEPTIDES; ALLATOSTATINS; NEUROSECRETORY CELLS; CORPUS CARDIACUM CORPUS ALLATUM COMPLEX; DIPLOPTERA-PUNCTATA; CALLIPHORA-VOMITORIA; (INSECTA);

D O I：

10.1007/BF00318770

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Neuronal circuits in the brain and retrocerebral complex of the cockroach Diploptera punctata have been mapped immunocytochemically with antisera directed against the extended enkephalin, Met-enkephalin-Arg6-Gly7-Leu8 (Met-8). The pathways link median and lateral neurosecretory cells with the corpus cardiacum/corpus allatum complex. In females, nerve fibres penetrate the corpora allata and varicosities or terminals, immunoreactive to Met-8, surround the glandular cells. Males differ in having almost no Met-8 immunoreactivity in the corpora allata. The corpora cardiaca of both males and females are richly supplied with Met-8 immunoreactive material, in particular in the 'cap' regions immediately adjacent to the corpora allata. A similarity in the amino-acid sequences of Met-8 and the C-terminus of the recently characterised allatostatins of D. punctata suggests that the pathways identified with the Met-8 antisera may be the same as those by which the allatostatins are transported from the brain to the corpus allatum. In comparative studies on the blowfly Calliphora vomitoria, similar neuronal pathways have been identified except that no sexual dimorphism with respect to amounts of immunoreactive material within the corpus allatum has been observed. These results suggest a possible homology in the neuropeptide regulation of the gland.

引用

页码：285 / 291

页数：7

共 19 条

[11]

REMY C, 1988, SOC EXPT BIOL SEMINA, V33, P43

[12]

RUEGG RP, 1983, EXPERIENTIA, V39, P1329

[13]

STERNBERGER LA, 1974, IMMUNOCYTOCHEMISTRY

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