A ONE-STEP ENZYME-IMMUNOASSAY FOR HUMAN MANGANESE SUPEROXIDE-DISMUTASE WITH MONOCLONAL-ANTIBODIES

被引：6

作者：

ADACHI, T

HIRANO, K

HAYASHI, K

MUTO, Y

OKUNO, F

机构：

[1] Department of Pharmaceutocs, Gifu Pharmaceutical University, Gifu, 502, 6-1

[2] First Department of Internal Medicine, Gifu University, School of Medicine, Gifu

[3] Department of Internal Medicine, Gihoku Hospital, Gifu

来源：

FREE RADICAL BIOLOGY AND MEDICINE | 1990年 / 8卷 / 01期

关键词：

Clinical analysis; Enzyme immunoassay; Free radicals; Horseradish peroxidase; Manganese superoxide dismutase; Monoclonal antibody;

D O I：

10.1016/0891-5849(90)90141-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A one-step enzyme immunoassay for the determination of manganese superoxide dismutase in serum has been developed with two kinds of monoclonal antibodies. Proposed method had high sensitivity (assay range, 0.4-200 ng/ml), good recovery (recovery percentage, 102.9-106.2%) and reproducibility (intraassay, C.V. = 1.87-3.66%; interassay, C.V. = 3.03-10.4%). From these results, it is possible to apply this method to routine clinical analysis and biochemical research with various purposes. © 1990.

引用

页码：25 / 31

页数：7

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