LOW-LEVEL ELECTROCHEMICAL DETECTION OF GLUCOSE-OXIDASE AND A GLUCOSE-OXIDASE CONJUGATE

被引：1

作者：

ALLEN, RM ^{[1
]}

BENNETTO, HP ^{[1
]}

LEWIS, IAS ^{[1
]}

MASON, JR ^{[1
]}

WESTRA, AR ^{[1
]}

机构：

[1] UNIV LONDON KINGS COLL,DEPT CHEM,LONDON W8 7AH,ENGLAND

来源：

BIOSENSORS & BIOELECTRONICS | 1995年 / 10卷 / 6-7期

关键词：

GLUCOSE OXIDASE; PLATINIZED CARBON PAPER ELECTRODE; AMPEROMETRY; HYDROGEN PEROXIDE; GLUCOSE OXIDASE CONJUGATE; ENZYME LABEL;

D O I：

10.1016/0956-5663(95)96938-U

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

An amperometric sensor for glucose oxidase is described. Partial optimization of the working electrode potential at 0.4 V vs. Ag/AgCl, 0.1 M KCl at a glucose concentration of 30 mM allows a low detection limit of 50 pM glucose oxidase within 25 min. The sensor exhibits a high degree of precision and reproducibility, and the same electrode may be re-used for many successive quantitative determinations. A mechanism for the sensor is proposed, in which hydrogen peroxide generated by glucose oxidase in bulk solution is oxidized at the working electrode, generating a current response that can be related to the glucose oxidase concentration. Conjugation of glucose oxidase with a coumarin-based pesticide analogue does not affect the amperometric response.

引用

页码：621 / 631

页数：11

共 7 条

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