CONSTRUCTION AND CHARACTERIZATION OF A YEAST ARTIFICIAL CHROMOSOME LIBRARY CONTAINING 5 HAPLOID SUGAR-BEET (BETA-VULGARIS L) GENOME EQUIVALENTS

被引：4
|
作者：
DELFAVERO, J [1 ]
WEYENS, G [1 ]
JACOBS, M [1 ]
EDWARDS, KE [1 ]
VAUTERIN, M [1 ]
机构：
[1] ICI AGROCHEM,JEALOTTS HILL RES STN,PLANT BIOTECHNOL SECT,ZENECA SEEDS,BRACKNELL RG12 6EY,BERKS,ENGLAND
来源：
THEORETICAL AND APPLIED GENETICS | 1994年 / 88卷 / 3-4期
关键词：
BETA VULGARIS; POLYMERASE CHAIN REACTION; YEAST ARTIFICIAL CHROMOSOME LIBRARY; GENOME MAPPING; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;
D O I：
10.1007/BF00223659
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
A yeast artificial chromosome (YAC) genomic library of Bera vulgaris was constructed in the pYAC4 vector. High-molecular-weight DNA was prepared from agarose-embedded leaf protoplasts from a triploid cultivar. The library was found to contain 33,500 clones in an ordered array of microtiter plates. Mean size of the inserts was estimated to be 135 kb, and the library should therefore represent the equivalent of five haploid genomes. The library was characterised for the presence of highly repetitive, chloroplast and single-copy sequences. In order to isolate single-copy sequences, 18 pools of DNA, each from 1920 individual YAC clones, were prepared for rapid screening of the library by the polymerase chain reaction. The results of these screenings showed that the number of isolated clones was at or near the frequency expected.
引用
收藏
页码：449 / 453
页数：5
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