HUMAN TAF(II)30 IS PRESENT IN A DISTINCT TFIID COMPLEX AND IS REQUIRED FOR TRANSCRIPTIONAL ACTIVATION BY THE ESTROGEN-RECEPTOR

被引：357

作者：

JACQ, X

BROU, C

LUTZ, Y

DAVIDSON, I

CHAMBON, P

TORA, L

机构：

[1] Laboratoire de Génétique Moléculaire des Eucaryotes, Centre National de la Recherche Scientifique, Institut de Chimie Biologique Faculté de Médecine, 67085 Strasbourg Cédex, 11, rue Humann

[2] Unité 184, l'Institut National de la Santé, la Recherche Médicale Institut, 67085 Strasbourg Cédex, 11, rue Humann

来源：

CELL | 1994年 / 79卷 / 01期

关键词：

D O I：

10.1016/0092-8674(94)90404-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We showed previously that coactivators mediating stimulation by different activators were associated with the TATA-binding protein (TBP) in distinct TFIID complexes. We have characterized a human TBP-associated factor (TAF), hTAF(II)30, associated with a subset of TFIID complexes. hTAF(II)30 interacts with the AF-2-containing region E of the human estrogen receptor (ER), but not with ER AF-1 or VP16. An antibody against hTAF(II)30 inhibited transcriptional stimulation by the ER AF-2 without affecting basal or VP16-activated transcription and allowed the separation of TFIID complex(es) containing hTAF(II)30 from complexes mediating the activity of VP16. These results directly demonstrate the existence of functionally dis tinct TFIID populations that share common TAF(II)s but differ in specific TAF(II)s.

引用

页码：107 / 117

页数：11

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