STRUCTURE OF CLONAL AND POLYCLONAL CELL ARRAYS IN CHIMERIC MOUSE RETINA

被引:58
作者
WILLIAMS, RW
GOLDOWITZ, D
机构
[1] Dept. of Anatomy and Neurobiology, University of Tennessee, College of Medicine, Memphis, TN 38163
关键词
CELL LINEAGE; CLONE; CHIMERA; CELL DETERMINATION;
D O I
10.1073/pnas.89.4.1184
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
One of the most striking results of recent cell-lineage studies of vertebrate retina is the marked variability in the size and types of clones marked by retroviral transfection and dye injection of embryonic progenitor cells. Is this variability due to microenvironmental modulation of cell determination, to lineage restriction, or to experimental perturbation of the progenitor cells? We have taken advantage of species-specific DNA probes to mark groups of lineage-related cells in experimental mouse chimeras. This method of marking cells has two distinct advantages over previous methods: direct manipulation of progenitor cells is avoided, and clones are established at an earlier stage of retinal development. The most notable feature of retinal cohorts in chimeras is their structural uniformity-each is a solid radial array that contains the same ratio of major cell types as the retina itself. This is true even of the smallest monoclonal cohorts, which contain fewer than 200 cells. Our result provides compelling empirical support for the hypothesis that the murine retina is made up of hundreds of relatively homogeneous radial units, each derived from single retinal precursor cells. This finding is inconsistent with micro-environmental modulation of clone structure early in development. We raise the possibility that the heterogeneity among clones marked by dye injection and transfection is due to progressive lineage restriction or to experimental perturbation of the retinal progenitor cells.
引用
收藏
页码:1184 / 1188
页数:5
相关论文
共 26 条
[1]  
CAJAL SRY, 1973, VERTEBRATE RETINA, P775
[2]   CELLULAR DNA REARRANGEMENTS AND EARLY DEVELOPMENTAL ARREST CAUSED BY DNA INSERTION IN TRANSGENIC MOUSE EMBRYOS [J].
COVARRUBIAS, L ;
NISHIDA, Y ;
TERAO, M ;
DEUSTACHIO, P ;
MINTZ, B .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (06) :2243-2247
[3]   EARLY EVENTS IN INSECT NEUROGENESIS .2. THE ROLE OF CELL-INTERACTIONS AND CELL LINEAGE IN THE DETERMINATION OF NEURONAL PRECURSOR CELLS [J].
DOE, CQ ;
GOODMAN, CS .
DEVELOPMENTAL BIOLOGY, 1985, 111 (01) :206-219
[4]  
FRORIEP A, 1906, HDB VERGLEICHENDEN E, P139
[5]   CELL ALLOCATION IN MAMMALIAN CNS FORMATION - EVIDENCE FROM MURINE INTERSPECIES AGGREGATION CHIMERAS [J].
GOLDOWITZ, D .
NEURON, 1989, 3 (06) :705-713
[6]  
GOLDOWITZ D, 1982, J NEUROSCI, V2, P1474
[7]   CELLULAR DETERMINATION IN THE XENOPUS RETINA IS INDEPENDENT OF LINEAGE AND BIRTH DATE [J].
HOLT, CE ;
BERTSCH, TW ;
ELLIS, HM ;
HARRIS, WA .
NEURON, 1988, 1 (01) :15-26
[8]   DENOVO METHYLATION AND EXPRESSION OF RETROVIRAL GENOMES DURING MOUSE EMBRYOGENESIS [J].
JAHNER, D ;
STUHLMANN, H ;
STEWART, CL ;
HARBERS, K ;
LOHLER, J ;
SIMON, I ;
JAENISCH, R .
NATURE, 1982, 298 (5875) :623-628
[9]   ARTIFACTS CAUSED BY CELL MICROINJECTION [J].
MILLER, DS ;
LAU, YT ;
HOROWITZ, SB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (05) :1426-1430
[10]   SPECIFICATION OF CEREBRAL CORTICAL AREAS [J].
RAKIC, P .
SCIENCE, 1988, 241 (4862) :170-176