KINETIC-MODEL OF DISACCHARIDE OXIDATION BY AGROBACTERIUM-TUMEFACIENS

被引：4

作者：

WALTER, J ^{[1
]}

JORDENING, HJ ^{[1
]}

机构：

[1] TECH UNIV CAROLO WILHELMINA BRAUNSCHWEIG,INST TECHNOL KOHLENHYDRATE,INST SUGAR,D-38106 BRAUNSCHWEIG,GERMANY

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1995年 / 48卷 / 01期

关键词：

HEXOPYRANOSIDE; CYTOCHROME C OXIDOREDUCTASE; DISACCHARIDE OXIDATION; OXIDATION; KINETIC MODEL; FERMENTATION;

D O I：

10.1002/bit.260480104

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Disaccharides were microbially transformed to their corresponding 3-keto-derivatives by resting cells of Agrobacterium tumefaciens NCPPB 396. The kinetics and yield of this highly specific oxidation depend on several factors. The oxygen concentration especially has a major influence on the production of 8-keto-derivatives and was investigated kinetically with respect to low stationary oxygen concentrations in solution. Experiments showed unconventional results that conflicted with normal Michaelis-Menten kinetics. Akinetic model was developed and the kinetic constants were calculated. The model and experimental data for sucrose, maltose, isomaltulose (palatinose), and leucrose are in good agreement with each other. Initial reaction rates with different sugars using constant oxygen concentrations resulted in a Michaelis-Menten type function. The complete kinetics, including the effect of disaccharide and oxygen concentrations, are presented. (C) 1995 John Wiley & Sons, Inc.

引用

页码：12 / 16

页数：5

共 20 条

[1] MICROBIOLOGICAL FORMATION AND PREPARATION OF 3-KETOGLYCOSIDES FROM DISACCHARIDES [J].