High-speed centrifugation induces aggregation of extracellular vesicles

被引:378
作者
Linares, Romain [1 ]
Tan, Sisareuth [1 ]
Gounou, Celine [1 ]
Arraud, Nicolas [1 ]
Brisson, Alain R. [1 ,2 ]
机构
[1] Univ Bordeaux, Mol Imaging & NanoBioTechnol, Pessac, France
[2] Inst Univ France, Paris, France
关键词
extracellular vesicles; blood plasma; cryo-electron microscopy; immuno-gold electron microscopy; flow cytometry; vesicle aggregation; centrifugation;
D O I
10.3402/jev.v4.29509
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Plasma and other body fluids contain cell-derived extracellular vesicles (EVs), which participate in physiopathological processes and have potential biomedical applications. In order to isolate, concentrate and purify EVs, high-speed centrifugation is often used. We show here, using electron microscopy, receptor-specific gold labelling and flow cytometry, that high-speed centrifugation induces the formation of EV aggregates composed of a mixture of EVs of various phenotypes and morphologies. The presence of aggregates made of EVs of different phenotypes may lead to erroneous interpretation concerning the existence of EVs harbouring surface antigens from different cell origins.
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页数:7
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