HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF TAURINE IN BIOLOGICAL-FLUIDS BY POSTCOLUMN FLUORESCENCE REACTION WITH THIAMINE

被引：10

作者：

YOKOYAMA, T ^{[1
]}

KINOSHITA, T ^{[1
]}

机构：

[1] KITASATO UNIV,SCH PHARMACEUT SCI,MINATO KU,TOKYO 108,JAPAN

来源：

JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 568卷 / 01期

关键词：

SAMPLES; REAGENT;

D O I：

10.1016/0378-4347(91)80355-G

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A high-performance liquid chromatographic method is described for the selective determination of taurine in biological fluids by post-column fluorescence reaction. Taurine was separated on an adsorption-distribution type Shodex Ionpac KC-811 column. Then it was converted with hypochlorite into the corresponding N-chloramine, which was allowed to react with thiamine to give fluorescent thiochrome. As little as 6 ng per injection of taurine could be determined. The average recoveries of spiked taurine in serum and urine were 99.5 +/- 2.7 and 101.8 2.9%, respectively. The method could be applied to the assay of taurine in human serum and urine with simple pretreatment.

引用

页码：212 / 218

页数：7

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