HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF TAURINE IN BIOLOGICAL-FLUIDS BY POSTCOLUMN FLUORESCENCE REACTION WITH THIAMINE

被引:10
作者
YOKOYAMA, T [1 ]
KINOSHITA, T [1 ]
机构
[1] KITASATO UNIV,SCH PHARMACEUT SCI,MINATO KU,TOKYO 108,JAPAN
来源
JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS | 1991年 / 568卷 / 01期
关键词
SAMPLES; REAGENT;
D O I
10.1016/0378-4347(91)80355-G
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A high-performance liquid chromatographic method is described for the selective determination of taurine in biological fluids by post-column fluorescence reaction. Taurine was separated on an adsorption-distribution type Shodex Ionpac KC-811 column. Then it was converted with hypochlorite into the corresponding N-chloramine, which was allowed to react with thiamine to give fluorescent thiochrome. As little as 6 ng per injection of taurine could be determined. The average recoveries of spiked taurine in serum and urine were 99.5 +/- 2.7 and 101.8 2.9%, respectively. The method could be applied to the assay of taurine in human serum and urine with simple pretreatment.
引用
收藏
页码:212 / 218
页数:7
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