PROTEIN SYNTHESIS IN SUBCELLULAR FRACTIONS OF BLOWFLY DURING DIFFERENT DEVELOPMENTAL STAGES

Protein synthesis by ribosomal preparations of the blowfly, Calliphora erythrocephala, has been measured in vitro in different developmental periods. There are two maxima, one at 1 to 2 days and one at 5 days after pupation. The polysomal profiles also show considerable changes; in 6- to 7-day-old larvae most of the particles are in the form of heavy polysomes, in later stages the reverse is seen. The protein/RNA quotients of the different preparations are high in the larvae, decrease 3 days after pupation and rise again 5 to 7 days after pupation. © 1968.