ENGINEERING PROTEINS FOR PURIFICATION

被引：110

作者：

SASSENFELD, HM

机构：

[1] H. M. Sassenfeld is at Immunex, Seattle, WA 98101

来源：

TRENDS IN BIOTECHNOLOGY | 1990年 / 8卷 / 04期

关键词：

D O I：

10.1016/0167-7799(90)90145-N

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Over the past decade, a new protein purification technique has emerged as a result of recombinant DNA technology. DNA, encoding additional polypeptide or protein tags, is fused to the gene of interest. Expression of these gene fusions results in protein fusions which may be purified by techniques using the properties of the additional polypeptide tag. This has eliminated the need for extensive screening and optimization procedures previously required for purification. © 1990.

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页码：88 / 93

页数：6

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