NEW PROCEDURE FOR PURIFYING HISTONE PAIRS H2A + H2B AND H-3 + H-4 FROM CHROMATIN USING HYDROXYLAPATITE

被引：318

作者：

SIMON, RH

FELSENFELD, G

机构：

[1] Laboratory of Molecular Biology, National Institute of Arthritis, Metabolism and Digestive Diseases, National Institutes of Health, Bethesda

来源：

NUCLEIC ACIDS RESEARCH | 1979年 / 6卷 / 02期

关键词：

D O I：

10.1093/nar/6.2.689

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A method to purify histone groups H2A+H2B and H3+H4 using dissociation with NaCl and hydroxylapatite chromatography is presented. The procedure 1s simple, involves mild solvents, and provides milligram quantities of histones of high purity. The histone pairs prepared by this method can regenerate chromatin-like characteristics when combined and reconstituted with DNA. © 1979 Information Retrieval Limited.

引用

页码：689 / 696

页数：8

共 16 条

[1]

ALFAGEME CR, 1974, J BIOL CHEM, V249, P729

[2] SYNTHESIS OF GLOBIN RIBONUCLEIC-ACID FROM DUCK RETICULOCYTE CHROMATIN IN-VITRO [J].

AXEL, R ;

CEDAR, H ;

FELSENFELD, G .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1973, 70 (07) :2029-2032

[3] SPECIFIC FOLDING AND CONTRACTION OF DNA BY HISTONES H-3 AND H-4 [J].

BINASTEIN, M ;

SIMPSON, RT .

CELL, 1977, 11 (03) :609-618

[4]

BLOOM KS, 1978, J BIOL CHEM, V253, P4446

[5] PHYSICAL STUDIES OF CHROMATIN - RECOMBINATION OF HISTONES WITH DNA [J].

BOSELEY, PG ;

BRADBURY, EM ;

BUTLERBROWNE, GS ;

CARPENTER, BG ;

STEPHENS, RM .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1976, 62 (01) :21-31

[6]

Camerini-Otero R D, 1978, Cold Spring Harb Symp Quant Biol, V42 Pt 1, P57

[7] ORGANIZATION OF HISTONES AND DNA IN CHROMATIN - EVIDENCE FOR AN ARGININE-RICH HISTONE KERNEL [J].

CAMERINIOTERO, RD ;

SOLLNERWEBB, B ;

FELSENFELD, G .

CELL, 1976, 8 (03) :333-347

[8] SUPERCOILING ENERGY AND NUCLEOSOME FORMATION - ROLE OF ARGININE-RICH HISTONE KERNEL [J].

CAMERINIOTERO, RD ;

FELSENFELD, G .

NUCLEIC ACIDS RESEARCH, 1977, 4 (05) :1159-1181

[9] CHROMATOGRAPHY OF CALF-THYMUS NUCLEOPROTEIN ON HYDROXYAPATITE COLUMNS [J].

FAULHABER, I ;

BERNARDI, G .

BIOCHIMICA ET BIOPHYSICA ACTA, 1967, 140 (03) :561-+

[10]

GIANNONI G, 1963, BIOCHIM BIOPHYS ACTA, V68, P157

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