A COMPARATIVE-STUDY OF LIPID COMPOSITIONS OF CRYPTOSPORIDIUM-PARVUM (APICOMPLEXA) AND MADIN-DARBY BOVINE KIDNEY-CELLS

被引：26
作者：
MITSCHLER, RR
WELTI, R
UPTON, SJ
机构：
[1] Division of Biology, Kansas State University, Manhattan, Kansas, 66506, Ackert Hall
来源：
JOURNAL OF EUKARYOTIC MICROBIOLOGY | 1994年 / 41卷 / 01期
关键词：
PARASITE LIPIDS;
D O I：
10.1111/j.1550-7408.1994.tb05927.x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Membrane lipid compositions of Cryptosporidium parvum and Madin-Darby bovine kidney cells, an epithelial-like cell line commonly used to study coccidia in vitro, were analyzed using both thin-layer chromatography and gas-liquid chromatography. Phosphatidylcholine was the predominant lipid in both C. parvum and Madin-Darby bovine kidney cells, comprising 65% and 41% of the total phospholipids, respectively. Phospholipids of C. parvum contained twice the level of 16:0 and twenty-fold more 18:2 than the Madin-Darby bovine kidney cell line. We suggest that the parasite may be capable of sequestering specific complex membrane lipids at concentrations greater than those in the host cells. This study constitutes the first report of the lipid composition of C. parvum.
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页码：8 / 12
页数：5
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